Blood Sample Collecting and Processing for Perio VAL Cohort

Authors

Makaylan H. Tseng, University of the PacificFollow
Alice Lin, Arthur A. Dugoni School of DentistryFollow
Nicole Thompson, Arthur A. Dugoni School of DentistryFollow
Jakob Einhaus, Stanford University School of MedicineFollow
Amy S. Tsai, Stanford University School of Medicine
Thomas A. Bonham, Stanford University School of MedicineFollow
Edward A. Ganio, Stanford University School of Medicine
Kazou Ando, Stanford University School of MedicineFollow
William Choi, Stanford University School of MedicineFollow
Karl C. Bruckman, Stanford University School of MedicineFollow
David Ojcius, Arthur A. Dugoni School of DentistryFollow
Dyani Gaudilliere, Stanford University School of MedicineFollow
Brice Gaudilliere, Stanford University School of MedicineFollow
Xiaoyuan Han, Arthur A. Dugoni School of DentistryFollow

Poster Number

20B

Lead Author Affiliation

Biological Sciences

Lead Author Status

Undergraduate - Junior

Second Author Affiliation

Biomedical Sciences

Second Author Status

Doctoral Student

Third Author Affiliation

Biomedical Sciences

Third Author Status

Doctoral Student

Fourth Author Affiliation

Anesthesiology

Fourth Author Status

Doctoral Student

Fifth Author Affiliation

Surgery

Fifth Author Status

Doctoral Student

Sixth Author Affiliation

Anesthesiology

Sixth Author Status

Staff

Additional Authors

Makaylan H. Tseng, Department of Biological Sciences, University of the Pacific, Undergraduate - Junior

Alice Lin, Department of Biomedical Sciences, University of the Pacific, Graduate Student

Nicole Thompson, Department of Biomedical Sciences, University of the Pacific, Graduate Student

Jakob Einhaus, Stanford University School of Medicine, Department of Anesthesiology, MD, PhD Student

Amy S. Tsai, Stanford University, School of Medicine, Department of Surgey, MD Resident

Thomas A. Bonham, Stanford University School of Medicine, Department of Anesthesiology, MS Staff

Edward A. Ganio, Stanford University School of Medicine, Department of Anesthesiology, PhD Staff

Kazou Ando, Stanford University School of Medicine, Department of Anesthesiology, MD, PhD, Faculty

William Choi, Stanford University School of Medicine, Department of Surgery, DDS, Faculty

Karl C. Bruckman, Stanford University School of Medicine, Department of Surgery, MD, DDS, Faculty

David Ojcius, Department of Biomedical Sciences, University of the Pacific, PhD, Faculty

Dyani Gaudilliere, Stanford University School of Medicine, Department of Surgery, DDS, Faculty

Brice Gaudilliere, Stanford University School of Medicine, Department of Anesthesiology, MD, PhD, Faculty

Xiaoyuan Han, Department of Biomedical Sciences, University of the Pacific, PhD, Faculty

Research or Creativity Area

Dentistry

Abstract

Introduction

Chronic periodontitis (ChP) is a prevalent inflammatory disease affecting 46% of the US population. ChP produces a profound local inflammatory response to dysbiotic oral microbiota that leads to the destruction of alveolar bone and tooth loss. ChP is also associated with systemic illnesses, including cardiovascular diseases, malignancies, and adverse pregnancy outcomes. The mechanisms underlying oral and systemic diseases are poorly understood. Our previous cohort demarcates systemic and cell-specific immune dysfunctions in patients with ChP, which can be temporarily reversed by the local treatment of ChP . Here, we conducted a validation cohort (Perio VAL) to validate our previous results.

Method

We consent 16 patients and 12 healthy controls in the Bell Dental Center (San Leandro, CA). Whole-blood samples were collected at baseline, 3 weeks, and 3 months after standard nonsurgical treatment for ChP. Blood was left unstimulated or stimulated with Porphyromonas gingivalis lipopolysaccharide (PgLPS), tumor necrosis factor α (TNFα), interleukins 12 (IL-12), and a cytokine cocktail containing interleukins 2/4/6 (IL-2/4/6) and stored at -80 degree. Before CyTOF analysis on white blood cells, samples were lysed to remove red blood cells.

Result

A total of about 420 samples were lysed and stored for CyTOF analysis.

Conclusion

We prepared the samples in Perio VAL cohort for the CyTOF analysis. In the future, we will conduct the multiplex single-cell analysis to investigate the oral and systemic immunological link in ChP.

Location

Don and Karen DeRosa University Center (DUC) Poster Hall

Start Date

27-4-2024 10:30 AM

End Date

27-4-2024 12:30 PM