Cloing and Expression of Thioredoxin Reductase from the Human Protozoal Parasite Trichomonas vaginalis

Poster Number

51

Lead Author Major

Biological Sciences

Format

Poster Presentation

Faculty Mentor Name

Kirkwood Land

Faculty Mentor Department

Biological Sciences

Additional Faculty Mentor Name

Lisa Wrischnik

Abstract/Artist Statement

Trichomonas vaginalis is a protozoal parasite that causes trichomoniasis in humans. The current FDA approved drugs for treatment of human trichomoniasis are metronidazole and tinidazole. Current data from the US Centers for Disease Control and Prevention have suggested that almost 5% of all clinical cases of trichomoniasis show some level of resistance to these drugs. In search of new chemotherapies, we have focused on the role of thioredoxin reductase, an enzyme that plays a role in susceptibility to metronidazole. To begin to understand the possible role of these enzymes, we have cloned and have begun to express the thioredoxin reductase from T. vaginalis. Our goal is to better understand the role of this enzyme in drug susceptibility and resistance.

Location

Grave Covell

Start Date

21-4-2012 10:00 AM

End Date

21-4-2012 12:00 PM

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Apr 21st, 10:00 AM Apr 21st, 12:00 PM

Cloing and Expression of Thioredoxin Reductase from the Human Protozoal Parasite Trichomonas vaginalis

Grave Covell

Trichomonas vaginalis is a protozoal parasite that causes trichomoniasis in humans. The current FDA approved drugs for treatment of human trichomoniasis are metronidazole and tinidazole. Current data from the US Centers for Disease Control and Prevention have suggested that almost 5% of all clinical cases of trichomoniasis show some level of resistance to these drugs. In search of new chemotherapies, we have focused on the role of thioredoxin reductase, an enzyme that plays a role in susceptibility to metronidazole. To begin to understand the possible role of these enzymes, we have cloned and have begun to express the thioredoxin reductase from T. vaginalis. Our goal is to better understand the role of this enzyme in drug susceptibility and resistance.