Cloning and Expression of an Aspartic Protease from the Human Protozoal Parasite Trichomonas vaginalis
Poster Number
46
Format
Poster Presentation
Faculty Mentor Name
Kirkwood Land
Faculty Mentor Department
Biological Sciences
Abstract/Artist Statement
Trichomonas vaginalis is a protozoal parasite of humans; and one mechanism of pathogenesis of this parasite is thought to be mediated by proteases. Inhibitors against aspartic proteases significantly blocked growth of T.vaginalis, and our present study is to clone and express the single aspartic protease. Using the genome project, we have succesfully cloned the full- length TvCatD gene from strain T1 into a protein expression vector for expression in E.coli. The purification of characterization of the recombinant enzyme will help us to design improved inhibitors targeting this critical enzyme.
Location
Grave Covell
Start Date
21-4-2012 10:00 AM
End Date
21-4-2012 12:00 PM
Cloning and Expression of an Aspartic Protease from the Human Protozoal Parasite Trichomonas vaginalis
Grave Covell
Trichomonas vaginalis is a protozoal parasite of humans; and one mechanism of pathogenesis of this parasite is thought to be mediated by proteases. Inhibitors against aspartic proteases significantly blocked growth of T.vaginalis, and our present study is to clone and express the single aspartic protease. Using the genome project, we have succesfully cloned the full- length TvCatD gene from strain T1 into a protein expression vector for expression in E.coli. The purification of characterization of the recombinant enzyme will help us to design improved inhibitors targeting this critical enzyme.