Presentation Category
Endo
Introduction/Context/Diagnosis
Objective: This study aims to test the hypothesis efficacy of 4 different irrigation systems against Enterococcus faecalis biofilm in a large root canal model (50.04) in extracted maxillary molar teeth. Materials & Method: This study used Maxillary human molar palatal canals (n=60) instrumented to a standard 50/.04 taper and autoclaved. Canals were infected with Enterococcus faecalis for 21 days. Teeth were randomly divided into 4 groups (n=15) and disinfected with group 1, standard needle irrigation (SNI); group, 2 ultrasonic activation (PUI); group 3, photothermal disinfection with indocyanine green (LEAP/L); or multi-sonic irrigation system (Gentlewave/GW). Bacterial reduction from the root canals was calculated based on pre- and post-disinfection samples. 5 teeth were selected randomly from each group and sectioned at the coronal, middle and apical third. Dentine specimens were stained using live/dead bacteria stain and analyzed using confocal laser scanning microscopy (CLSM). Three random locations on the root were scanned for a total of 9 images per tooth (3 per section) resulting in 180 images total, to determine the bacterial viability and depth of irrigation penetration within the dentinal tubules. Results: Quantitative analysis demonstrated a bacterial reduction of: SNI (99.1%, SD 1.77), PUI (99.1%, SD 1.24), LEAP (99.6%, SD 0.58), and Gentlewave (99.4%, SD 0.77). CLSM results are still pending. Conclusion: All four disinfection methods successfully reduced the number of viable bacteria within the palatal canal.
Location
Arthur A Dugoni School of Dentistry, 155 5th St, San Francisco, CA 94103, USA
Format
Presentation
Comparison of Four Disinfection Techniques Using a Large Root Canal Model
Arthur A Dugoni School of Dentistry, 155 5th St, San Francisco, CA 94103, USA
Objective: This study aims to test the hypothesis efficacy of 4 different irrigation systems against Enterococcus faecalis biofilm in a large root canal model (50.04) in extracted maxillary molar teeth. Materials & Method: This study used Maxillary human molar palatal canals (n=60) instrumented to a standard 50/.04 taper and autoclaved. Canals were infected with Enterococcus faecalis for 21 days. Teeth were randomly divided into 4 groups (n=15) and disinfected with group 1, standard needle irrigation (SNI); group, 2 ultrasonic activation (PUI); group 3, photothermal disinfection with indocyanine green (LEAP/L); or multi-sonic irrigation system (Gentlewave/GW). Bacterial reduction from the root canals was calculated based on pre- and post-disinfection samples. 5 teeth were selected randomly from each group and sectioned at the coronal, middle and apical third. Dentine specimens were stained using live/dead bacteria stain and analyzed using confocal laser scanning microscopy (CLSM). Three random locations on the root were scanned for a total of 9 images per tooth (3 per section) resulting in 180 images total, to determine the bacterial viability and depth of irrigation penetration within the dentinal tubules. Results: Quantitative analysis demonstrated a bacterial reduction of: SNI (99.1%, SD 1.77), PUI (99.1%, SD 1.24), LEAP (99.6%, SD 0.58), and Gentlewave (99.4%, SD 0.77). CLSM results are still pending. Conclusion: All four disinfection methods successfully reduced the number of viable bacteria within the palatal canal.
Comments/Acknowledgements
Presentation Category: Residents: Endodontics