Presentation Category
Endo
Introduction/Context/Diagnosis
Objective: This study aims to test the efficacy of 4 different irrigation systems against Enterococcus faecalis biofilm in a small root canal model. Materials & Methods: Distobuccal canals of human maxillary molars were instrumented to a standard 20/.04 taper and autoclaved. Canals were then infected with E. faecalis for 21 days. Teeth were randomly divided into 4 groups (15 teeth/group) and disinfected with either standard needle irrigation (SNI), ultrasonic activation (PUI), LEAP (L), or Gentlewave (GW). Bacterial reduction from the root canals was calculated based on pre- and post-disinfection samples. 5 teeth were selected randomly from each group and sectioned at the coronal, middle and apical third. Dentin specimens were stained using live/dead bacteria stain and analyzed using confocal laser scanning microscopy (CLSM). Results: All S2 samples showed significant bacterial reduction compared to S1 (P<0.05). Quantitative analysis demonstrated a bacterial reduction of: SNI (98.6%), PUI (99.2%), L (97.9%), and GW (97.8%). CLSM demonstrated various bacterial penetration capability among the coronal, middle and apical segments of the canals. Conclusion: All four disinfection methods successfully reduced the number of viable bacteria within the distobuccal canal.
Location
Arthur A Dugoni School of Dentistry, 155 5th St, San Francisco, CA 94103, USA
Format
Presentation
Comparison of Four Disinfection Techniques Using a Small Root Canal Model
Arthur A Dugoni School of Dentistry, 155 5th St, San Francisco, CA 94103, USA
Objective: This study aims to test the efficacy of 4 different irrigation systems against Enterococcus faecalis biofilm in a small root canal model. Materials & Methods: Distobuccal canals of human maxillary molars were instrumented to a standard 20/.04 taper and autoclaved. Canals were then infected with E. faecalis for 21 days. Teeth were randomly divided into 4 groups (15 teeth/group) and disinfected with either standard needle irrigation (SNI), ultrasonic activation (PUI), LEAP (L), or Gentlewave (GW). Bacterial reduction from the root canals was calculated based on pre- and post-disinfection samples. 5 teeth were selected randomly from each group and sectioned at the coronal, middle and apical third. Dentin specimens were stained using live/dead bacteria stain and analyzed using confocal laser scanning microscopy (CLSM). Results: All S2 samples showed significant bacterial reduction compared to S1 (P<0.05). Quantitative analysis demonstrated a bacterial reduction of: SNI (98.6%), PUI (99.2%), L (97.9%), and GW (97.8%). CLSM demonstrated various bacterial penetration capability among the coronal, middle and apical segments of the canals. Conclusion: All four disinfection methods successfully reduced the number of viable bacteria within the distobuccal canal.
Comments/Acknowledgements
Presentation Category: Residents: Endodontics