Cost-Efficient Rotary Bioreactor with Specialized Tissue Culture Vessel and CO2 Monitoring System
Format
SOECS Senior Project
Faculty Mentor Name
Shadi Othman
Faculty Mentor Department
Bioengineering Department, School of Engineering and Computer Science
Additional Faculty Mentor Name
Robert Halliwell
Additional Faculty Mentor Department
Pharmacy Department
Additional Faculty Mentor Name
Chi-Wook Lee
Additional Faculty Mentor Department
Mechanical Engineering Department, School of Engineering and Computer Science
Abstract/Artist Statement
The growth of tissues for in vitro applications is a scientific process that has shown great interest in the research community. Commercially static bioreactors are often used to produce viable 3D tissue culture by keeping the cells within the medium alive. This process involves generating a controlled environment inside a cell-culture chamber for the management and growth of cells. However, while commercially available static bioreactors are used to culture a large variety of cells, they are very expensive to manufacture and not suited to running small experimental trials. To address this, we developed a user-friendly, versatile, and cost-efficient bioreactor that induces mechanical stimulus designed to promote cell proliferation.
Temperature and CO2 are regulated through a complex electromechanical system with real-time online tracking and data collection, allowing users to easily maintain and control their microenvironment. Petri dishes and cell culture flasks can be placed on a rotating platform that aids in the homogenous distribution of media and is managed to incorporate multiple different cell lines. Successful validation experiments of the bioreactor’s microenvironment can be expanded to the analysis of 3D in-vitro models and effects of infectious diseases, which can benefit medical professionals, students, and the public alike from more methods of studying in-vivo systems.
Location
Chambers Technology Center, 3601 Pacific Ave, Stockton, CA 95211, USA
Start Date
6-5-2023 2:30 PM
End Date
6-5-2023 4:30 PM
Cost-Efficient Rotary Bioreactor with Specialized Tissue Culture Vessel and CO2 Monitoring System
Chambers Technology Center, 3601 Pacific Ave, Stockton, CA 95211, USA
The growth of tissues for in vitro applications is a scientific process that has shown great interest in the research community. Commercially static bioreactors are often used to produce viable 3D tissue culture by keeping the cells within the medium alive. This process involves generating a controlled environment inside a cell-culture chamber for the management and growth of cells. However, while commercially available static bioreactors are used to culture a large variety of cells, they are very expensive to manufacture and not suited to running small experimental trials. To address this, we developed a user-friendly, versatile, and cost-efficient bioreactor that induces mechanical stimulus designed to promote cell proliferation.
Temperature and CO2 are regulated through a complex electromechanical system with real-time online tracking and data collection, allowing users to easily maintain and control their microenvironment. Petri dishes and cell culture flasks can be placed on a rotating platform that aids in the homogenous distribution of media and is managed to incorporate multiple different cell lines. Successful validation experiments of the bioreactor’s microenvironment can be expanded to the analysis of 3D in-vitro models and effects of infectious diseases, which can benefit medical professionals, students, and the public alike from more methods of studying in-vivo systems.