Title

Analysis of a Kex2-like Gene in the Protozoal Parasite Trichomonas vaginalis

Poster Number

49

Lead Author Major

Biological Sciences

Format

Poster Presentation

Faculty Mentor Name

Kirkwood Land

Faculty Mentor Department

Biological Sciences

Additional Faculty Mentor Name

Lisa Wrischnik

Abstract/Artist Statement

Trichomonas vaginalis is a protozoal parasite that causes trichomoniasis in humans. The mechanism of pathogenesis is thought to involve proteases. In this study, we have focused on the cloning and expression of a kex2-like serine protease. In related parasites, like malaria and trypanosomes, kex2-like serine proteases play a role in processing virulence factors. To begin to understand the possible role of TvKex2, we have cloned and isolated the gene, and have begun to express recombinant forms of Tvkex2. We have also started cloning the gene into a yeast vector pYES for genetic complementation studies of kex2 mutants of Saccharomyces cerevisiae.

Location

Grave Covell

Start Date

21-4-2012 10:00 AM

End Date

21-4-2012 12:00 PM

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Apr 21st, 10:00 AM Apr 21st, 12:00 PM

Analysis of a Kex2-like Gene in the Protozoal Parasite Trichomonas vaginalis

Grave Covell

Trichomonas vaginalis is a protozoal parasite that causes trichomoniasis in humans. The mechanism of pathogenesis is thought to involve proteases. In this study, we have focused on the cloning and expression of a kex2-like serine protease. In related parasites, like malaria and trypanosomes, kex2-like serine proteases play a role in processing virulence factors. To begin to understand the possible role of TvKex2, we have cloned and isolated the gene, and have begun to express recombinant forms of Tvkex2. We have also started cloning the gene into a yeast vector pYES for genetic complementation studies of kex2 mutants of Saccharomyces cerevisiae.