Analysis of a Kex2-like Gene in the Protozoal Parasite Trichomonas vaginalis
Poster Number
49
Format
Poster Presentation
Faculty Mentor Name
Kirkwood Land
Faculty Mentor Department
Biological Sciences
Additional Faculty Mentor Name
Lisa Wrischnik
Abstract/Artist Statement
Trichomonas vaginalis is a protozoal parasite that causes trichomoniasis in humans. The mechanism of pathogenesis is thought to involve proteases. In this study, we have focused on the cloning and expression of a kex2-like serine protease. In related parasites, like malaria and trypanosomes, kex2-like serine proteases play a role in processing virulence factors. To begin to understand the possible role of TvKex2, we have cloned and isolated the gene, and have begun to express recombinant forms of Tvkex2. We have also started cloning the gene into a yeast vector pYES for genetic complementation studies of kex2 mutants of Saccharomyces cerevisiae.
Location
Grave Covell
Start Date
21-4-2012 10:00 AM
End Date
21-4-2012 12:00 PM
Analysis of a Kex2-like Gene in the Protozoal Parasite Trichomonas vaginalis
Grave Covell
Trichomonas vaginalis is a protozoal parasite that causes trichomoniasis in humans. The mechanism of pathogenesis is thought to involve proteases. In this study, we have focused on the cloning and expression of a kex2-like serine protease. In related parasites, like malaria and trypanosomes, kex2-like serine proteases play a role in processing virulence factors. To begin to understand the possible role of TvKex2, we have cloned and isolated the gene, and have begun to express recombinant forms of Tvkex2. We have also started cloning the gene into a yeast vector pYES for genetic complementation studies of kex2 mutants of Saccharomyces cerevisiae.