Title

Cloning and Expression of an Aspartic Protease from the Human Protozoal Parasite Trichomonas vaginalis

Poster Number

46

Lead Author Major

Biological Sciences

Format

Poster Presentation

Faculty Mentor Name

Kirkwood Land

Faculty Mentor Department

Biological Sciences

Abstract/Artist Statement

Trichomonas vaginalis is a protozoal parasite of humans; and one mechanism of pathogenesis of this parasite is thought to be mediated by proteases. Inhibitors against aspartic proteases significantly blocked growth of T.vaginalis, and our present study is to clone and express the single aspartic protease. Using the genome project, we have succesfully cloned the full- length TvCatD gene from strain T1 into a protein expression vector for expression in E.coli. The purification of characterization of the recombinant enzyme will help us to design improved inhibitors targeting this critical enzyme.

Location

Grave Covell

Start Date

21-4-2012 10:00 AM

End Date

21-4-2012 12:00 PM

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Apr 21st, 10:00 AM Apr 21st, 12:00 PM

Cloning and Expression of an Aspartic Protease from the Human Protozoal Parasite Trichomonas vaginalis

Grave Covell

Trichomonas vaginalis is a protozoal parasite of humans; and one mechanism of pathogenesis of this parasite is thought to be mediated by proteases. Inhibitors against aspartic proteases significantly blocked growth of T.vaginalis, and our present study is to clone and express the single aspartic protease. Using the genome project, we have succesfully cloned the full- length TvCatD gene from strain T1 into a protein expression vector for expression in E.coli. The purification of characterization of the recombinant enzyme will help us to design improved inhibitors targeting this critical enzyme.