Document Type
Article
Publication Title
Cell Reports
ISSN
2211-1247
Volume
7
Issue
5
DOI
10.1016/j.celrep.2014.04.043
First Page
1434
Last Page
1442
Publication Date
6-12-2014
Abstract
Transcription factors have recently been shown to colocalize in hotspot regions of the genome, which are further clustered into super-enhancers. However, the detailed molecular organization of transcription factors at hotspot regions is poorly defined. Here, we have used digital genomic footprinting to precisely define factor localization at a genome-wide level during the early phase of 3T3-L1 adipocyte differentiation, which allows us to obtain detailed molecular insight into how transcription factors target hotspots. We demonstrate the formation of ATF-C/EBP heterodimers at a composite motif on chromatin, and we suggest that this may be a general mechanism for integrating external signals on chromatin. Furthermore, we find evidence of extensive recruitment of transcription factors to hotspots through alternative mechanisms not involving their known motifs and demonstrate that these alternative binding events are functionally important for hotspot formation and activity. Taken together, these findings provide a framework for understanding transcription factor cooperativity in hotspots. © 2014 The Authors.
Recommended Citation
Siersbæk, R.,
Baek, S.,
Rabiee, A.,
Nielsen, R.,
Traynor, S.,
Clark, N.,
Sandelin, A.,
Jensen, O.,
Sung, M.,
Hager, G. L.,
&
Mandrup, S.
(2014).
Molecular architecture of transcription factor hotspots in early adipogenesis.
Cell Reports, 7(5), 1434–1442.
DOI: 10.1016/j.celrep.2014.04.043
https://scholarlycommons.pacific.edu/phs-facarticles/450
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
