Document Type
Article
Publication Title
Cell Reports
ISSN
2211-1247
Volume
7
Issue
5
DOI
10.1016/j.celrep.2014.04.042
First Page
1443
Last Page
1455
Publication Date
6-12-2014
Abstract
It is becoming increasingly clear that transcription factors operate in complex networks through thousands of genomic binding sites, many of which bind several transcription factors. However, the extent and mechanisms of crosstalk between transcription factors at these hotspots remain unclear. Using a combination of advanced proteomics and genomics approaches, we identify ~12,000 transcription factor hotspots (~400bp) in the early phase of adipogenesis, and we find evidence of both simultaneous and sequential binding of transcription factors at these regions. We demonstrate that hotspots are highly enriched in large super-enhancer regions (several kilobases), which drive the early adipogenic reprogramming of gene expression. Our results indicate that cooperativity between transcription factors at the level of hotspots as well as super-enhancers is very important for enhancer activity and transcriptional reprogramming. Thus, hotspots and super-enhancersconstitute important regulatory hubs that serve to integrate external stimuli on chromatin. © 2014 The Authors.
Recommended Citation
Siersbæk, R.,
Rabiee, A.,
Nielsen, R.,
Sidoli, S.,
Traynor, S.,
Loft, A.,
Poulsen, L. L.,
Rogowska-Wrzesinska, A.,
Jensen, O. N.,
&
Mandrup, S.
(2014).
Transcription factor cooperativity in early adipogenic hotspots and super-enhancers.
Cell Reports, 7(5), 1443–1455.
DOI: 10.1016/j.celrep.2014.04.042
https://scholarlycommons.pacific.edu/phs-facarticles/449
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
