A Rational Approach for Creating Peptides Mimicking Antibody Binding

Authors

Sameer Sachdeva, University of the Pacific
Hyun Joo, University of the PacificFollow
Jerry Tsai, University of the Pacific
Bhaskara Jasti, University of the PacificFollow
Xiaoling Li, University of the PacificFollow

Document Type

Article

Publication Title

Scientific Reports

ISSN

2045-2322

Volume

9

Issue

1

DOI

10.1038/s41598-018-37201-6

First Page

997

Last Page

997

Publication Date

1-30-2019

Abstract

This study reports a novel method to design peptides that mimic antibody binding. Using the Knob-Socket model for protein-protein interaction, the interaction surface between Cetuximab and EGFR was mapped. EGFR binding peptides were designed based on geometry and the probability of the mapped knob-sockets pairs. Designed peptides were synthesized and then characterized for binding specificity, affinity, cytotoxicity of drug-peptide conjugate and inhibition of phosphorylation. In cell culture studies, designed peptides specifically bind and internalize to EGFR overexpressing cells with three to four-fold higher uptake compared to control cells that do not overexpress EGFR. The designed peptide, Pep11, bound to EGFR with K

Recommended Citation

Sachdeva, S., Joo, H., Tsai, J., Jasti, B., & Li, X. (2019). A Rational Approach for Creating Peptides Mimicking Antibody Binding. Scientific Reports, 9(1), 997–997. DOI: 10.1038/s41598-018-37201-6
https://scholarlycommons.pacific.edu/phs-facarticles/393

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.