Presentation Category
Endo
Introduction/Context/Diagnosis
Objective: This study aims to assess the efficacy of LEAP (L) systems against Enterococcus faecalis biofilm in three root canal size (20/04,30/04 and 50/04) models in comparison to standard needle irrigation (SNI). Materials & Methods: Distobuccal roots of maxillary molars, single-canal mandibular premolars and palatal roots of maxillary first molars were instrumented to size 20/.04, 30/.04 and 50/.04 respectively and autoclaved. Canals where then incubated with E. faecalis for 21 days. Teeth were randomly divided into 2 groups (15 teeth/group) and disinfected with either SNI or L. Bacterial culture sampling was done for all specimens. 5 teeth were selected randomly from each group and sectioned at the coronal, middle and apical third, stained using live/dead bacteria stain and analyzed using confocal laser scanning microscopy (CLSM). Results: All S2 samples showed significant bacterial reduction compared to S1 (P< 0.05). The bacterial reduction in SNI and L was 98.6% and 97.9% in the canals prepared to size 20/04; 98.3% and 98.0% in the canals prepared to size 30/04 and 99.1% and 99.6% in canals prepared to size 50/04 respectively .CLSM analysis demonstrated various degree of root canal disinfection at the different canal levels. Conclusion: Both disinfection methods allowed for reduced the number of viable bacteria in all three canal preparation sizes.
Location
Arthur A Dugoni School of Dentistry, 155 5th St, San Francisco, CA 94103, USA
Format
Presentation
Understanding the Photothermal Energy of LEAP in Different Canal Size Models
Arthur A Dugoni School of Dentistry, 155 5th St, San Francisco, CA 94103, USA
Objective: This study aims to assess the efficacy of LEAP (L) systems against Enterococcus faecalis biofilm in three root canal size (20/04,30/04 and 50/04) models in comparison to standard needle irrigation (SNI). Materials & Methods: Distobuccal roots of maxillary molars, single-canal mandibular premolars and palatal roots of maxillary first molars were instrumented to size 20/.04, 30/.04 and 50/.04 respectively and autoclaved. Canals where then incubated with E. faecalis for 21 days. Teeth were randomly divided into 2 groups (15 teeth/group) and disinfected with either SNI or L. Bacterial culture sampling was done for all specimens. 5 teeth were selected randomly from each group and sectioned at the coronal, middle and apical third, stained using live/dead bacteria stain and analyzed using confocal laser scanning microscopy (CLSM). Results: All S2 samples showed significant bacterial reduction compared to S1 (P< 0.05). The bacterial reduction in SNI and L was 98.6% and 97.9% in the canals prepared to size 20/04; 98.3% and 98.0% in the canals prepared to size 30/04 and 99.1% and 99.6% in canals prepared to size 50/04 respectively .CLSM analysis demonstrated various degree of root canal disinfection at the different canal levels. Conclusion: Both disinfection methods allowed for reduced the number of viable bacteria in all three canal preparation sizes.
Comments/Acknowledgements
Presentation Category: Residents: Endodontics