Traditional approaches and recent tools for studying inflammasome activity

Authors

Cássio Luiz C. Almeida-da-Silva, University of the PacificFollow
Aline C. de Abreu Moreira-Souza, University of the PacificFollow
David M. Ojcius, University of the PacificFollow

ORCiD

David Ojcius: https://orcid.org/0000-0003-1461-4495

Department

Biomedical Sciences

Document Type

Article

Publication Title

Journal of Immunological Methods

ISSN

1872-7905

Volume

533

Issue

1

DOI

10.1016/j.jim.2024.113744

First Page

1

Last Page

9

Publication Date

8-16-2024

Abstract

Inflammasomes play a major role in the immune response to infection, development of autoimmune disease, and control of cancer. Western blots were originally used in the early 2000s to characterize inflammasome activation. Since then, a panoply of techniques has been developed to characterize and visualize inflammasome activation in cells, tissues, and animals. This review article describes the most common techniques used by researchers in the inflammasome field and proposes that cell-specific characterization of inflammasome activation in tissues or animals may soon be commonly reported.

Recommended Citation

Almeida-da-Silva, C. C., de Abreu Moreira-Souza, A. C., & Ojcius, D. M. (2024). Traditional approaches and recent tools for studying inflammasome activity. Journal of Immunological Methods, 533(1), 1–9. DOI: 10.1016/j.jim.2024.113744
https://scholarlycommons.pacific.edu/dugoni-facarticles/873