Title

Expression of recombinant proteins in Pichia pastoris

ORCiD

Nejat Düzgüneş: 0000-0001-6159-1391

Document Type

Article

Publication Title

Applied Biochemistry and Biotechnology

ISSN

0273-2289

Volume

142

Issue

2

DOI

10.1007/s12010-007-0003-x

First Page

105

Last Page

124

Publication Date

8-1-2007

Abstract

Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression. These elements include vectors, host strains, heterologous gene integration into the genome, secretion factors, and the glycosylation profile. In particular, we discuss and evaluate the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins. Optimization can be achieved by controlling the medium composition, pH, temperature, and dissolved oxygen, as well as by methanol induction and feed mode. © Humana Press Inc. 2007.

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