Expression of recombinant proteins in Pichia pastoris
ORCiD
Nejat Düzgüneş: 0000-0001-6159-1391
Department
Biomedical Sciences
Document Type
Article
Publication Title
Applied Biochemistry and Biotechnology
ISSN
0273-2289
Volume
142
Issue
2
DOI
10.1007/s12010-007-0003-x
First Page
105
Last Page
124
Publication Date
8-1-2007
Abstract
Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression. These elements include vectors, host strains, heterologous gene integration into the genome, secretion factors, and the glycosylation profile. In particular, we discuss and evaluate the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins. Optimization can be achieved by controlling the medium composition, pH, temperature, and dissolved oxygen, as well as by methanol induction and feed mode. © Humana Press Inc. 2007.
Recommended Citation
Li, P.,
Anumanthan, A.,
Gao, X.,
Ilangovan, K.,
Suzara, V. V.,
Düzgüneş, N.,
&
Renugopalakrishnan, V.
(2007).
Expression of recombinant proteins in Pichia pastoris.
Applied Biochemistry and Biotechnology, 142(2), 105–124.
DOI: 10.1007/s12010-007-0003-x
https://scholarlycommons.pacific.edu/dugoni-facarticles/540
