ORCID
Joseph Harrison: 0000-0002-2118-6524
Document Type
Article
Publication Title
PLoS Biology
Department
Chemistry
ISSN
1544-9173
Volume
18
Issue
12
DOI
10.1371/journal.pbio.3000975
Publication Date
12-11-2020
Abstract
The anaphase-promoting complex/cyclosome (APC/C) is an E3 ubiquitin ligase and critical regulator of cell cycle progression. Despite its vital role, it has remained challenging to globally map APC/C substrates. By combining orthogonal features of known substrates, we predicted APC/C substrates in silico. This analysis identified many known substrates and suggested numerous candidates. Unexpectedly, chromatin regulatory proteins are enriched among putative substrates, and we show experimentally that several chromatin proteins bind APC/C, oscillate during the cell cycle, and are degraded following APC/C activation, consistent with being direct APC/C substrates. Additional analysis revealed detailed mechanisms of ubiquitylation for UHRF1, a key chromatin regulator involved in histone ubiquitylation and DNA methylation maintenance. Disrupting UHRF1 degradation at mitotic exit accelerates G1-phase cell cycle progression and perturbs global DNA methylation patterning in the genome. We conclude that APC/C coordinates crosstalk between cell cycle and chromatin regulatory proteins. This has potential consequences in normal cell physiology, where the chromatin environment changes depending on proliferative state, as well as in disease. Copyright:
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Franks, J. L.,
Martinez-Chacin, R. C.,
Wang, X.,
Tiedemann, R. L.,
Bonacci, T.,
Choudhury, R.,
Bolhuis, D. L.,
Enrico, T. P.,
Mouery, R. D.,
Damrauer, J. S.,
Yan, F.,
Harrison, J. S.,
Major, M. B.,
Hoadley, K. A.,
Suzuki, A.,
Rothbart, S. B.,
Brown, N. G.,
&
Emanuele, M. J.
(2020).
In silico APC/C substrate discovery reveals cell cycle-dependent degradation of UHRF1 and other chromatin regulators.
PLoS Biology, 18(12),
DOI: 10.1371/journal.pbio.3000975
https://scholarlycommons.pacific.edu/cop-facarticles/835
Comments
Funding Sponsor: National Institute of General Medical Sciences, R01GM120309