The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene

Authors

Eric Marcusson, University of California, San Diego
Bruce Horazdovsky, University of California, San Diego
Joan Lin-Cereghino, University of California, San DiegoFollow
Editte Gharakhanian, University of California, San Diego
Scott Emr, University of California, San Diego

Document Type

Article

Publication Title

Cell

Department

Biological Sciences

ISSN

0092-8674

Volume

77

Issue

4

DOI

10.1016/0092-8674(94)90219-4

First Page

579

Last Page

586

Publication Date

5-1-1994

Abstract

The S. cerevisiae VPS10 (vacuolar protein sorting) gene encodes a type I transmembrane protein of 1577 amino acids required for the sorting of the soluble vacuolar protein carboxypeptidase Y (CPY). Mutations in VPS10 result in the selective missorting and secretion of CPY; all other vacuolar proteins tested are delivered to the vacuole in vps10 mutants. Chemical cross-linking studies demonstrate that Vps10p and the Golgi-modified precursor form of CPY directly interact. A single amino acid change in the CPY vacuolar sorting signal prevents this interaction. Vps10p also interacts with a hybrid protein containing the CPY sorting signal fused to the normally secreted enzyme invertase. Subcellular fractionation indicates that the majority of Vps10p is localized to a late Golgi compartment where vacuolar proteins are sorted. We propose that VPS10 encodes a CPY sorting receptor that executes multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment.

Recommended Citation

Marcusson, E., Horazdovsky, B., Lin-Cereghino, J., Gharakhanian, E., & Emr, S. (1994). The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene. Cell, 77(4), 579–586. DOI: 10.1016/0092-8674(94)90219-4
https://scholarlycommons.pacific.edu/cop-facarticles/738