New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris.

Authors

Geoff P. Lin-Cereghino, Oregon Graduate Institute of Science and TechnologyFollow
Joan Lin-Cereghino, Oregon Graduate Institute of Science and TechnologyFollow
Anthony Sunga, Oregon Graduate Institute of Science and Technology
Monique Johnson, Oregon Graduate Institute of Science and Technology
May Lim, Oregon Graduate Institute of Science and Technology
Martina Gleeson, Invitrogen
James Cregg, Oregon Graduate Institute of Science and TechnologyFollow

Document Type

Article

Publication Title

Gene

Department

Biological Sciences

ISSN

0378-1119

Volume

263

Issue

1--2

DOI

10.1016/S0378-1119(00)00576-X

First Page

159

Last Page

169

Publication Date

1-24-2001

Abstract

We describe the isolation and characterization of three new biosynthetic genes-ARG4, ADE1, and URA3-from the methylotrophic yeast Pichia pastoris. The predicted products of the genes share significant sequence similarity to their Saccharomyces cerevisiae counterparts, namely argininosuccinate lyase, PR-aminoimidazolesuccinocarboxamide synthase, and orotidine-5′-phosphate decarboxylase, respectively. Along with the previously described HIS4 gene, each gene was incorporated as the yeast selectable marker into a set of shuttle vectors designed to express foreign genes in P. pastoris. In addition, we have constructed a series of host strains containing all possible combinations of ade1, arg4, his4, and ura3 auxotrophies to be used with these new vectors.

Recommended Citation

Lin-Cereghino, G. P., Lin-Cereghino, J., Sunga, A., Johnson, M., Lim, M., Gleeson, M., & Cregg, J. (2001). New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris.. Gene, 263(1--2), 159–169. DOI: 10.1016/S0378-1119(00)00576-X
https://scholarlycommons.pacific.edu/cop-facarticles/732