New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris.

Document Type

Article

Publication Title

Gene

Department

Biological Sciences

ISSN

0378-1119

Volume

263

Issue

1--2

DOI

10.1016/S0378-1119(00)00576-X

First Page

159

Last Page

169

Publication Date

1-24-2001

Abstract

We describe the isolation and characterization of three new biosynthetic genes-ARG4, ADE1, and URA3-from the methylotrophic yeast Pichia pastoris. The predicted products of the genes share significant sequence similarity to their Saccharomyces cerevisiae counterparts, namely argininosuccinate lyase, PR-aminoimidazolesuccinocarboxamide synthase, and orotidine-5′-phosphate decarboxylase, respectively. Along with the previously described HIS4 gene, each gene was incorporated as the yeast selectable marker into a set of shuttle vectors designed to express foreign genes in P. pastoris. In addition, we have constructed a series of host strains containing all possible combinations of ade1, arg4, his4, and ura3 auxotrophies to be used with these new vectors.

Link to Full Text

Share

COinS