Cloning and characterization of the Pichia pastoris MET2 gene as a selectable marker

Authors

Der Thor, University of the PacificFollow
See Xiong, University of the Pacific
Claire C. Orazem, University of the Pacific
An-Chun Kwan, University of the Pacific
James Cregg, Keck Graduate Institute of Applied Life SciencesFollow
Joan Lin-Cereghino, University of the PacificFollow
Geoff P. Lin-Cereghino, University of the PacificFollow

Document Type

Article

Publication Title

Fems Yeast Research

Department

Biological Sciences

ISSN

1567-1356

Volume

5

Issue

10

DOI

10.1016/j.femsyr.2005.03.009

First Page

935

Last Page

942

Publication Date

7-1-2005

Abstract

We describe the isolation and characterization of a new biosynthetic gene, MET2, from the methylotrophic yeast Pichia pastoris. The predicted product of PpMET2 is significantly similar to its Saccharomyces cerevisiae counterpart, ScMET2, which encodes homoserine-O-transacetylase. The ScMET2 was able to complement the P. pastoris met2 strain; however, the converse was not true. Expression vectors based on PpMET2 for the intracellular and secreted production of foreign proteins and corresponding auxotrophic strains were constructed and tested for use in heterologous expression. The expression vectors and corresponding strains provide greater flexibility when using P. pastoris for recombinant protein expression.

Recommended Citation

Thor, D., Xiong, S., Orazem, C. C., Kwan, A., Cregg, J., Lin-Cereghino, J., & Lin-Cereghino, G. P. (2005). Cloning and characterization of the Pichia pastoris MET2 gene as a selectable marker. Fems Yeast Research, 5(10), 935–942. DOI: 10.1016/j.femsyr.2005.03.009
https://scholarlycommons.pacific.edu/cop-facarticles/727