Title

Vectors and strains for expression.

Document Type

Article

Publication Title

Pichia Protocols

Department

Biological Sciences

Volume

389

DOI

10.1007/978-1-59745-456-8_2

First Page

11

Last Page

25

Publication Date

1-1-2007

Abstract

Selection of both an appropriate expression vector and corresponding strain is crucial for successful expression of heterologous proteins in Pichia pastoris. This chapter explores both the standard and new vector/strain options available for protein expression in this yeast. Incorporated into expression vectors are selectable markers based on biosynthetic pathway genes, dominant drug resistance, or the P. pastoris formaldehyde dehydrogenase gene (FLD1). Novel strains available for expression include those that increase secretion of heterologous protein by overexpressing eukaryotic protein disulfide isomerase, and those that decrease hyperglycosylation or provide human-type glycosylation. This chapter also discusses methods to create multicopy strains that will potentially provide optimized expression of recombinant proteins in P. pastoris.

Comments

Part of the Methods in Molecular Biology book series (MIMB, volume 389)

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