Direct selection of Pichia pastoris expression strains using new G418 resistance vectors
Document Type
Article
Publication Title
Yeast
Department
Biological Sciences
ISSN
0749-503X
Volume
25
Issue
4
DOI
10.1002/yea.1587
First Page
293
Last Page
299
Publication Date
4-1-2008
Abstract
The methylotrophic yeast, Pichia pastoris, is widely used as a host organism for the expression of heterologous proteins. Currently, the Zeocin and blasticidin resistance genes are the only dominant selectable markers that can be used for primary selection of transformants. In this report we describe new expression vectors that can be used to select directly for P. pastoris transformants using G418 resistance conferred by a modified Tn903kanr gene. Compared to other dominant markers, this system is more economical and offers a higher transformation efficiency, due to the small sizes of the cloning vectors, pKAN B and pKANα B (GenBank Accession Nos EU285585 and EU285586, respectively). Additionally, multicopy transformants can be generated using these new vectors.
Recommended Citation
Lin-Cereghino, J.,
Hashimoto, M.,
Moy, A.,
Castelo, J.,
Orazem, C.,
Kuo, P.,
Xiong, S.,
Gandhi, V.,
Hatae, C.,
Chan, A.,
&
Lin-Cereghino, G. P.
(2008).
Direct selection of Pichia pastoris expression strains using new G418 resistance vectors.
Yeast, 25(4), 293–299.
DOI: 10.1002/yea.1587
https://scholarlycommons.pacific.edu/cop-facarticles/723
