Title

Mutant strains of Pichia pastoris with enhanced secretion of recombinant proteins

Document Type

Article

Publication Title

Biotechnology Letters

Department

Biological Sciences

ISSN

0141-5492

Volume

35

Issue

11

DOI

10.1007/s10529-013-1290-7

First Page

1925

Last Page

1935

Publication Date

11-1-2013

Abstract

Although Pichia pastoris is a popular protein expression system, it exhibits limitations in its ability to secrete heterologous proteins. Therefore, a REMI (restriction enzyme mediated insertion) strategy was utilized to select mutant beta-g alactosidase s upersecretion (bgs) strains that secreted increased levels of a β-galactosidase reporter. Many of the twelve BGS genes may have functions in intracellular signaling or vesicle transport. Several of these strains also appeared to contain a more permeable cell wall. Preliminary characterization of four bgs mutants showed that they differed in the ability to enhance the export of other reporter proteins. bgs13, which has a disruption in a gene homologous to Saccharomyces cerevisiae protein kinase C (PKC1), gave enhanced secretion of most recombinant proteins that were tested, raising the possibility that it has the universal super-secreter phenotype needed in an industrial production strain of P. pastoris.

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