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Date of Award

2009

Document Type

Thesis - Pacific Access Restricted

Degree Name

Master of Science (M.S.)

Department

Biological Sciences

First Advisor

Lisa A. Wrischnik

First Committee Member

Kirkwood Land

Second Committee Member

Gregg Jongeward

Abstract

Trichomonas vagina/is is a common sexually transmitted disease, affecting women more often than men. It has only been seen to undergo mitosis, even though published studies have confinned the organism has meiotic proteins. These meiotic proteins are known to function in other organisms with a key protein in homologous recombination, DMCl. RT-PCR analysis shows low expression ofDMCl in mitotically-growing cultures, and we found that some stresses on the organism increase DMCl expression. Polyclonal antibodies raised against DMCl protein have been used to test whole celllysates of the Tl and G3 strains of Trichomonas vagina/is but no obvious expression has been detected. We also used western blot analysis to show that superoxide dismutase is expressed in the standard lab strains Tl and G3 and immunocytochemistry studies showed that HA-tagged SOD6 protein localizes in the cytoplasm. Lastly, we found that SOD protein abundance increased in the CDC085 strain compared to Tl and G3, especially under aerobic conditions.

Pages

60

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