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Date of Award
2009
Document Type
Thesis - Pacific Access Restricted
Degree Name
Master of Science (M.S.)
Department
Biological Sciences
First Advisor
Lisa A. Wrischnik
First Committee Member
Kirkwood Land
Second Committee Member
Gregg Jongeward
Abstract
Trichomonas vagina/is is a common sexually transmitted disease, affecting women more often than men. It has only been seen to undergo mitosis, even though published studies have confinned the organism has meiotic proteins. These meiotic proteins are known to function in other organisms with a key protein in homologous recombination, DMCl. RT-PCR analysis shows low expression ofDMCl in mitotically-growing cultures, and we found that some stresses on the organism increase DMCl expression. Polyclonal antibodies raised against DMCl protein have been used to test whole celllysates of the Tl and G3 strains of Trichomonas vagina/is but no obvious expression has been detected. We also used western blot analysis to show that superoxide dismutase is expressed in the standard lab strains Tl and G3 and immunocytochemistry studies showed that HA-tagged SOD6 protein localizes in the cytoplasm. Lastly, we found that SOD protein abundance increased in the CDC085 strain compared to Tl and G3, especially under aerobic conditions.
Pages
60
Recommended Citation
Foray, Nathalie Emma-Marie. (2009). Characterization of TvDMC1 and TvSOD6 expression and function in trichomonas vaginalis. University of the Pacific, Thesis - Pacific Access Restricted. https://scholarlycommons.pacific.edu/uop_etds/721
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