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Date of Award
2004
Document Type
Thesis - Pacific Access Restricted
Degree Name
Master of Science (M.S.)
Department
Biological Sciences
First Advisor
Craig A. Vierra
First Committee Member
Geoff Lin-Cereghino
Second Committee Member
Lisa A. Wrischnik
Abstract
Activated B-cell Factor-I (ABF-1) is a class II basic helix-loop-helix protein expressed in activated B-cells, EBV -immortalized lymphoblastoid cell lines and embryonic skeletal muscle in mice. ABF -1 dimerizes with another bHLH protein E4 7 to form a transcriptional repressor ofE2A, which is a gene essential to the proper development of B- and T -cells. In an effort to study genes in B-cells regulated by ABF- 1, I have attempted to construct a Burkitt Lymphoma cell line allowing tetracycline regulated expression of ABF-1. The tetracycline repressor gene was first added to these cells, creating a parental line oftet repression. Next, both a full-length and a truncated version of ABF -1 were added to the cell line. The truncated version of the protein is expressed in the presence of tetracycline, but completely repressed in its absence, demonstrating a tightly-regulated system of inducible expression. The full-length version of ABF-1 has yet to be expressed in this cell line; however, it has been expressed in a HeLa cell line, demonstrating that the construct has been properly made.
Pages
37
Recommended Citation
Richmond, Jennifer Mary. (2004). Development of a Burkitt Lymphoma cell line with inducible expression of activated B-cell factor-1. University of the Pacific, Thesis - Pacific Access Restricted. https://scholarlycommons.pacific.edu/uop_etds/595
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