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Construction of an ABF-1 inducible expression cell line utilized to conduct a microarray analysis

Author

Ryan M. O'Connell, University of the Pacific

Date of Award

2001

Document Type

Thesis - Pacific Access Restricted

Degree Name

Master of Science (M.S.)

Department

Biological Sciences

First Advisor

Craig Vierra

First Committee Member

Gregg Jongeward

Second Committee Member

Geoffrey Lin-Cereghino

Abstract

ABF-1 is a human class ll basic helix-loop-helix transcription factor that is expressed predominately in EBV immortalized and activated B lymphocytes. A human cell line was stably transfected with a tetracycline regulated ABF-1 expression vector. The cell line revealed tight regulation of ABF-1 expression following stable incorporation of the vector into the genomic DNA. Upon induction of ABF-1 expression, the cell line exhibited a dramatic growth rate decrease. In order to monitor genes regulated by ABF-1, cells were collected both before and after induced ABF-1 expression and subjected to a microarray analysis. Early interpretations of the microarray data support the findings that ABF-1 may be regulating gene expression in a manner that facilitates withdrawal from the cell cycle.

Pages

148

Recommended Citation

O'Connell, Ryan M.. (2001). Construction of an ABF-1 inducible expression cell line utilized to conduct a microarray analysis. University of the Pacific, Thesis - Pacific Access Restricted. https://scholarlycommons.pacific.edu/uop_etds/556