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Date of Award

2000

Document Type

Thesis - Pacific Access Restricted

Degree Name

Master of Science (M.S.)

Department

Biological Sciences

First Advisor

Craig Vierra

First Committee Member

Dale W. McNeal

Second Committee Member

Gregg D. Jongeward

Abstract

The human ABF-1 gene is expressed in activated B-cells and Epstein-Barr virus immortalized lymphoblastoid cell lines. ABF-1 represents the only member belonging to the basic helix-loop-helix (bHLH) family of transcription factors whose expression pattern is restricted to B cells. ABF-1 forms heterodirner complexes with E2A to modulate gene transcription. Here we report the isolation often novel ABF-1 splice products from a human B-cell plasmacytoma cell line. Analysis of the nucleotide sequence revealed approximately five clones harboring changes within the coding region of the reported full-length ABF-1 eDNA. In some cases, these changes resulted in the elimination of the coding region specifying the bHLH domain and likely affect DNAbinding and dirnerization of ABF-1. For the other retrieved splice products, the changes within the eDNA were localized to the 3' untranslated region (UTR), which likely indicate the potential for translational regulation of the ABF -1 gene.

Pages

79

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