Date of Award
2020
Document Type
Thesis
Degree Name
Master of Science (M.S.)
Department
Biological Sciences
First Advisor
Eric Thomas
First Committee Member
Jerry Tsai
Second Committee Member
Melanie Felmlee
Abstract
Defensive mechanisms vary widely in the animal kingdom ranging from physical defenses like spines to chemical defenses such as toxins. Toxins in these secretions and tissues can fluctuate from enzymes to lipids to uncharacterized chemicals. Next generation -omics technology and mass spectrometry are extremely important in analyzing these samples because of their ability to distinguish minute amounts of toxic substance within a complicated sample. The goal of this experiment was to look at secretions and tissues from Corydoras sterbai. All samples in this study were proteolyzed using a mixture of Trypsin and Lys-C, fractionated, and run through nanoLC-MS/MS analysis using an Orbitrap Fusion™ Tribrid™ mass spectrometer. Using guanidine hydrochloride as a denaturant, a total of 420 database peptide matches were discovered in the secretions and up to 777 database peptide matches among the tissues. Proteins of interest found in both the secretion and in the hypothesized gland include members of the prostaglandin synthesis pathway, phospholipases, and peroxiredoxins. It is theorized that C. sterbai uses its serrated rays in addition to these proteins to defend themselves from would be predators.
Pages
52
Recommended Citation
Wictor, Erik Powell. (2020). A Proteomic Analysis of Corydoras Sterbai Secretions and Tissues. University of the Pacific, Thesis. https://scholarlycommons.pacific.edu/uop_etds/3700
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