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Date of Award
2015
Document Type
Thesis - Pacific Access Restricted
Degree Name
Master of Science (M.S.)
Department
Biological Sciences
First Advisor
Lisa Wrischnik
First Committee Member
William Chan
Second Committee Member
Douglas Weiser
Abstract
The aryl hydrocarbon receptor (AhR) carboxyl terminal transcriptional activation domain was cloned, purified in denatured conditions from bacteria, refolded via limited dialysis, and analyzed for proper refolding via co-immunoprecipitation with the known binding partner SRC-1. This AhR NΔ515 transactivation domain construct was used, along with amino terminal AhR deletion constructs AhR CΔ274 and AhR CΔ553, to attempt to elucidate the nature of the interaction between AhR and p23 in vitro.
Pages
101
ISBN
9781339047355
Recommended Citation
Thompson, John D.. (2015). Analysis of the interaction between the co-chaperone p23 and the aryl hydrocarbon receptor. University of the Pacific, Thesis - Pacific Access Restricted. https://scholarlycommons.pacific.edu/uop_etds/176
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