Testosterone Mediated Renal Monocarboxylate Transporter Expression
Poster Number
12B
Introduction/Abstract
The kidney is a major route of elimination with renal excretion a function of glomerular filtration, tubular secretion and tubular reabsorption. The extent of active tubular reabsorption and secretion of substrates is governed by transporters. Proton- and sodium- dependent monocarboxylate transporters (MCTs/SMCTs) mediate the trafficking of endogenous and exogenous monocarboxylates across renal tubule cells (1). Our laboratory aims to investigate sex and cross-sex hormone dependent differences in transporter expression. Prior studies demonstrated castrated male rats had greater renal MCT1 membrane expression than intact male and ovariectomized and intact females (2). Proestrus and metestrus female rats had lower renal MCT1 membrane expression relative to ovariectomized and females in other stages of the estrus cycle (2).
Purpose
The objective is to quantify MCT1, MCT4, SMCT1 and CD147 renal membrane expression in ovariectomized and castrated animal models administered testosterone or corresponding placebo.
Method
8-week-old ovariectomized (OVX) female and castrated (CST) male Sprague Dawley rats were received. 10-week-old rats were subcutaneously administered 7.5 mg testosterone or corresponding placebo 60-day release pellets. Kidney was collected following 21 days of treatment and the ProteoExtract Native Membrane Extraction (Milipore Sigma) kit was utilized to isolate membrane protein. Bicinchoninic acid (BCA) assay was used to determine total protein concentration for sample dilution. Western blot was used to validate membrane extraction and to quantify the proteins of interest.
Significance
Kidney transporter expression is a determinant of disposition and renal clearance. Thus, it is important to quantify relevant renal transporters and to evaluate the effects of sex and sex-hormone on expression.
Location
Library and Learning Center, 3601 Pacific Ave., Stockton, CA 95211
Format
Poster Presentation
Testosterone Mediated Renal Monocarboxylate Transporter Expression
Library and Learning Center, 3601 Pacific Ave., Stockton, CA 95211
The kidney is a major route of elimination with renal excretion a function of glomerular filtration, tubular secretion and tubular reabsorption. The extent of active tubular reabsorption and secretion of substrates is governed by transporters. Proton- and sodium- dependent monocarboxylate transporters (MCTs/SMCTs) mediate the trafficking of endogenous and exogenous monocarboxylates across renal tubule cells (1). Our laboratory aims to investigate sex and cross-sex hormone dependent differences in transporter expression. Prior studies demonstrated castrated male rats had greater renal MCT1 membrane expression than intact male and ovariectomized and intact females (2). Proestrus and metestrus female rats had lower renal MCT1 membrane expression relative to ovariectomized and females in other stages of the estrus cycle (2).
