Title

LOOKING INTO THE ROLE PLAYED BY ARYL HYDROCARBON RECEPTOR (AHR) IN COLON CARCINOMA TUMOR MODEL

Poster Number

7a

Lead Author Affiliation

Pharmaceutics and Medicinal Chemistry

Lead Author Status

Doctoral Student

Introduction

Aryl hydrocarbon receptor (AHR), commonly known as an environmental sensor involved in the metabolism and elimination of xenobiotic substances is also an important modulator in the development and functioning of the immune system. AHR expression is varied in the T cell subsets with the highest expression in T-helper 17 and T regulatory cells

Purpose

Work from many researchers has suggested that AHR can act as tumor promoter or a tumor suppressor depending on the tumor type. Our goal is to understand the role played by AHR in MC38 syngeneic colon carcinoma tumor model

Method

- MC38 (mouse colon carcinoma) tumor model. Used c57BL/6 mice - MC38 cells - Caliper and STUDY LOG software for measuring tumor burden - BD Fortessa for FACS analysis to look into the the tumor infiltrating lymphocytes - RT-PCR to look into the expression of genes activated when MC38 cells were exposed to TCDD

Results

In the absence of AHR, MC38 tumor progresses by an increase in tumor associated macrophages (TAMs), M2 macrophages and a decrease in CD8a positive cytotoxic lymphocytes. This has been assessed by pharmacologic blocking of the receptor using CH223191 and in AHR deficient (AHR-/-) mice

Significance

Therefore AHR acts as a tumor suppressor gene in colon carcinoma tumor model and silencing it may lead to colon cancer progression.

Location

DeRosa University Center

Format

Poster Presentation

Poster Session

Afternoon 1pm-3pm

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Apr 28th, 1:00 PM Apr 28th, 3:00 PM

LOOKING INTO THE ROLE PLAYED BY ARYL HYDROCARBON RECEPTOR (AHR) IN COLON CARCINOMA TUMOR MODEL

DeRosa University Center

Aryl hydrocarbon receptor (AHR), commonly known as an environmental sensor involved in the metabolism and elimination of xenobiotic substances is also an important modulator in the development and functioning of the immune system. AHR expression is varied in the T cell subsets with the highest expression in T-helper 17 and T regulatory cells