LOOKING INTO THE ROLE PLAYED BY ARYL HYDROCARBON RECEPTOR (AHR) IN COLON CARCINOMA TUMOR MODEL
Poster Number
7a
Introduction/Abstract
Aryl hydrocarbon receptor (AHR), commonly known as an environmental sensor involved in the metabolism and elimination of xenobiotic substances is also an important modulator in the development and functioning of the immune system. AHR expression is varied in the T cell subsets with the highest expression in T-helper 17 and T regulatory cells
Purpose
Work from many researchers has suggested that AHR can act as tumor promoter or a tumor suppressor depending on the tumor type. Our goal is to understand the role played by AHR in MC38 syngeneic colon carcinoma tumor model
Method
- MC38 (mouse colon carcinoma) tumor model. Used c57BL/6 mice - MC38 cells - Caliper and STUDY LOG software for measuring tumor burden - BD Fortessa for FACS analysis to look into the the tumor infiltrating lymphocytes - RT-PCR to look into the expression of genes activated when MC38 cells were exposed to TCDD
Results
In the absence of AHR, MC38 tumor progresses by an increase in tumor associated macrophages (TAMs), M2 macrophages and a decrease in CD8a positive cytotoxic lymphocytes. This has been assessed by pharmacologic blocking of the receptor using CH223191 and in AHR deficient (AHR-/-) mice
Significance
Therefore AHR acts as a tumor suppressor gene in colon carcinoma tumor model and silencing it may lead to colon cancer progression.
Location
DeRosa University Center
Format
Poster Presentation
Poster Session
Afternoon 1pm-3pm
LOOKING INTO THE ROLE PLAYED BY ARYL HYDROCARBON RECEPTOR (AHR) IN COLON CARCINOMA TUMOR MODEL
DeRosa University Center
Aryl hydrocarbon receptor (AHR), commonly known as an environmental sensor involved in the metabolism and elimination of xenobiotic substances is also an important modulator in the development and functioning of the immune system. AHR expression is varied in the T cell subsets with the highest expression in T-helper 17 and T regulatory cells
