LUCIFERASE GENE TRANSFECTION MEDIATED BY CATIONIC LIPOSOMES COMPRISING NOVEL TRANS-2-AMINOCYCLOHEXANOL-BASED AMPHIPHILES
Introduction/Abstract
Gene transfection is one of the most promising and rapidly developing methodologies in modern medicine. Gene material (DNA) is commonly delivered into cells in a form of complexes with liposomes (lipoplexes).
Purpose
Development of a novel type of acid-sensitive liposomes for targeted gene delivery.
Method
We suggested recently a novel type of pH-sensitive drug delivery system, “fliposomes”, which contain trans-2-aminocyclohexanol-based amphiphiles, “flipids”, that perform a conformational flip (a drastic change of molecular shape) and trigger an instant cargo release in acidic media. The present research has explored a possibility of application of fliposomes in gene delivery.
Results
We found that incorporation of trans-2-aminocyclohexanol-based amphiphiles as helper lipids in DOTAP-cationic liposomes enhances substantially the transfection of the Gaussia luciferase plasmid into HeLa and B16F1 cells. The efficiency of transfection strongly depends on the structure of lipophilic chains and NRR’ groups.
Significance
New type of acid-sensitive liposomes provides a prospective gene delivery system for application in biology and medicine.
Location
DeRosa University Center, Stockton campus, University of the Pacific
Format
Poster Presentation
LUCIFERASE GENE TRANSFECTION MEDIATED BY CATIONIC LIPOSOMES COMPRISING NOVEL TRANS-2-AMINOCYCLOHEXANOL-BASED AMPHIPHILES
DeRosa University Center, Stockton campus, University of the Pacific
Gene transfection is one of the most promising and rapidly developing methodologies in modern medicine. Gene material (DNA) is commonly delivered into cells in a form of complexes with liposomes (lipoplexes).
