CATIONIC LIPID COATED MAGESIUM PHOSPHATE NANOPARTICLE FOR PROTEIN DELIVERY
Introduction/Abstract
The safety and efficacy of therapeutic protein are limited by its in vitro and in vivo instability, immunogenicity and short half life. Protein delivery systems are designed to protect the protein from denaturation and destabilization, and eventually deliver it to the targeted site.
Purpose
To design and prepare a cationic lipid coated magnesium phosphate nanoparticle formulation (LMP NP) for efficient delivery of protein.
Method
The negatively charged magnesium phosphate (MgP) nanoparticle was prepared using water-in-oil microemulsions. The cargo protein catalase was entrapped into cationic liposome by lipid hydration and extrusion. After being mixed together, the cationic liposome carrying catalase engulfed the negatively charged MgP nanoparticles to form the catalase encapsulated cationic lipid coated MgP nanoparticles (LMP NP-catalase). The concentration of the MgP nanoparticles was measured by phosphorus assay. The size and zeta potential of the LMP NP-catalase formulation was measured by zetasizer. The pH - trigged protein release from the LMP NP-catalase formulation was determined by a hydrogen peroxide degradation assay, where the concentration of remaining hydrogen peroxide was measured by UV-Vis. The delivery of LMP NP-catalase formulation into MCF-7 cells was determined by the resultant resistance of the cells against hydrogen peroxide using the MTS cell viability assay.
Results
The resulting LMP NP-catalase formulation had an average diameter below 300nm, the zeta potential was around +40mV due to the cationic lipid. The LMP NP-catalase degraded hydrogen peroxide 4 times faster at pH 5 than pH 7.4. The LMP NP-catalase formulation had 82% recovery of viable MCF-7 cells after exposure to hydrogen peroxide
Significance
A new nanoparticle system for delivery of proteins into cells has been designed, prepared and characterized.
Location
DeRosa University Center, Stockton campus, University of the Pacific
Format
Poster Presentation
CATIONIC LIPID COATED MAGESIUM PHOSPHATE NANOPARTICLE FOR PROTEIN DELIVERY
DeRosa University Center, Stockton campus, University of the Pacific
The safety and efficacy of therapeutic protein are limited by its in vitro and in vivo instability, immunogenicity and short half life. Protein delivery systems are designed to protect the protein from denaturation and destabilization, and eventually deliver it to the targeted site.
