HOW TO CRACK YOUR GENETIC CODE FROM ONE DROP OF SALIVA
Introduction/Abstract
Molecular genetics is without any doubt an important diagnostic tool in medicine and rapidly becoming more and more used in dentistry. Many common conditions in dentistry have a specific genetic background: not only craniofacial anomalies, but also root resorption, periodontitis, hypodontia. Numerous efficient tests for specific single nucleotide polymorphisms are available. However, sometimes the very first step – to obtain a specimen suitable for DNA analysis - may close the door to clarify genetic diagnosis and thus help with treatment planning, prognostic assessment, and counseling about prevention. An excellent source for DNA analysis is the buccal epithelial cells that exfoliate from the inner epithelial linings of the oral cavity.
Purpose
To review techniques using buccal epithelial cells done by others and compare with our previous study.
Method
An extensive review of studies published in recent literature was performed and combined with results of our own studies to determine a kind of sample and the most suitable method of sample collection for genetic testing of dental patients.
Results
Intraoral collection of cells from buccal mucosa seems to be a non-invasive method of choice. Three methods of collection were considered: buccal swab, oral rinse and saliva collection. Saliva was shown to give the highest yield of DNA of a good quality. In our own study (Pitigoi-Aron et al., 2005) three methods of DNA isolation from saliva were tested: REDExtract-N-Ampa (Sigma) for fresh saliva, saliva dried on filter paper, and Oragene saliva kit. The most efficient and practical was the use of saliva dried on filter paper (safe for shipping and storage) and also giving a good yield of DNA for molecular genetic testing. Since that study, this method has been successfully used in our laboratory.
Significance
Collection of saliva is acceptable noninvasive technique that can be used safely in children and adults. We introduced and tested for several years relatively inexpensive protocol for collection, transport, and storage of saliva using dried saliva spots on filter paper that yield a good quality of DNA suitable for genetic analysis. This technique can be used on-site as well as off-site, in dental office, or even on medical missions.
Location
DeRosa University Center, Stockton campus, University of the Pacific
Format
Poster Presentation
HOW TO CRACK YOUR GENETIC CODE FROM ONE DROP OF SALIVA
DeRosa University Center, Stockton campus, University of the Pacific
Molecular genetics is without any doubt an important diagnostic tool in medicine and rapidly becoming more and more used in dentistry. Many common conditions in dentistry have a specific genetic background: not only craniofacial anomalies, but also root resorption, periodontitis, hypodontia. Numerous efficient tests for specific single nucleotide polymorphisms are available. However, sometimes the very first step – to obtain a specimen suitable for DNA analysis - may close the door to clarify genetic diagnosis and thus help with treatment planning, prognostic assessment, and counseling about prevention. An excellent source for DNA analysis is the buccal epithelial cells that exfoliate from the inner epithelial linings of the oral cavity.
