177390 ALOX12/15 AND COX2 IN HUMAN BM-MSC AND SCAP IN VITRO
Introduction/Abstract
Traditionally, human bone marrow-derived and mesenchymal stem cells from the dental apical papilla (BM-MSC and SCAP, respectively) are cultivated in normal atmospheric level of oxygen (21%). Their isolation and transfer to culture represents a shift from 2-8% in their niche to 21% in culture. Their re-implantation would mean a shift from 21% to 2-8% in the implantation site.
Purpose
Does the change from 21% to 5% oxygen influence physiological status of mesenchymal stem cells?
Method
Human BM-MSC and SCAP were freshly isolated (IRB approval Nr.09-99.1). Cells were grown in basal medium from Lonza, 10% human adult serum, 2mM L-glutamin and antibiotics. Gene expression of COX2, ALOX 12 and 15 was measured by RT PCR (SABiosciences, Applied Biosystems) following 21 to 5% and 5 to 21% transfers.
Results
Following 5 to 21% transfer, no significant change in gene expression was observed. Transfer 21 to 5% led to increased expression of COX2 in BM-MSC. Transfer 21 to 5% led to increased expression of ALOX12 and 15 in SCAP.
Significance
The increase in ALOX12/15 and COX2 gene expression may be a part of a cellular reaction to hypoxia. We recommend that a tissue level of oxygen is maintained during isolation and expansion of human BM-MSC and SCAP in vitro.
Location
DeRosa University Center, Stockton campus, University of the Pacific
Format
Poster Presentation
177390 ALOX12/15 AND COX2 IN HUMAN BM-MSC AND SCAP IN VITRO
DeRosa University Center, Stockton campus, University of the Pacific
Traditionally, human bone marrow-derived and mesenchymal stem cells from the dental apical papilla (BM-MSC and SCAP, respectively) are cultivated in normal atmospheric level of oxygen (21%). Their isolation and transfer to culture represents a shift from 2-8% in their niche to 21% in culture. Their re-implantation would mean a shift from 21% to 2-8% in the implantation site.
