GROWTH PROMOTION OF HUMAN BM-MSC AND DP-MSC BY PLATELET FACTORS IN NORMAL OR LOW OXYGEN IN VITRO
Introduction/Abstract
Activated platelets and low oxygen are two out of many factors involved in wound healing. One of their effects in early stages of wound healing is stimulation of growth of cells involved in repair or regeneration. It has been shown that mesenchymal stem cells cultivated in growth medium supplemented with serum containing platelet-derived factors and maintained in a low-oxygen atmosphere exhibited an increased proliferation rate in comparison with controls.
Purpose
We were interested, if effects of platelet factors on human bone marrow-derived (BMSC) and dental pulp-derived (DPSC) mesenchymal stem cells are similar and if they are identical in atmosphere containing normal or low oxygen concentrations.
Method
Human BMSC were purchased from Lonza. Human DPSC were isolated from healthy third molars (IRB approval 09-99.1). Growth medium consisted of basal medium (Lonza) with 10% human serum, 2mM L-glutamin, 100 units/mL penicillin G and 100 μg/mL streptomycin. Serum and platelet-derived factors were prepared from human venous blood. Biospherix equipment maintained atmosphere with 5% oxygen and 5% carbon dioxide.
Results
Growth stimulatory effects of platelet-derived factors were greater in DPSC than in BMSC. Growth-stimulatory effect of platelet derived factors was more expressed in atmosphere containing 21% oxygen than in 5% oxygen level.
Significance
It seems that cultured human DPSC are more responsive to growth-promoting influence of platelet-derived factors and low oxygen than cultured human BMSC.
Location
DeRosa University Center, Stockton campus, University of the Pacific
Format
Poster Presentation
GROWTH PROMOTION OF HUMAN BM-MSC AND DP-MSC BY PLATELET FACTORS IN NORMAL OR LOW OXYGEN IN VITRO
DeRosa University Center, Stockton campus, University of the Pacific
Activated platelets and low oxygen are two out of many factors involved in wound healing. One of their effects in early stages of wound healing is stimulation of growth of cells involved in repair or regeneration. It has been shown that mesenchymal stem cells cultivated in growth medium supplemented with serum containing platelet-derived factors and maintained in a low-oxygen atmosphere exhibited an increased proliferation rate in comparison with controls.
