Genomic evaluation of morphological variation in Variovorax paradoxus EPS
Poster Number
2C
Research or Creativity Area
Natural Sciences
Abstract
Variovorax paradoxus is a species of versatile bacteria with a remarkable capacity for degrading organic pollutants, presenting promising opportunities for bioremediation applications. Their ability to metabolize various environmental contaminants, such as pesticides and aromatic compounds, highlights their potential role in mitigating pollution and restoring ecological balance. Understanding the mechanisms underlying their degradation pathways is crucial for harnessing their bioremediation capabilities effectively. In this research, we applied various methodologies to observe the genomic characteristics across accessible sequences of Variovorax species.
A previously grown colony was mixed into yeast extract for inoculation. After giving it time to grow in the shaking incubator for a few days, we took the liquid cultures and plated them onto freshwater medium. Allowing it to incubate, we then streaked and restreaked the colonies several times to ensure that each plate involved only one bacterial type (per plate). To determine the different colony types found amongst our plates, the Echo revolve microscope was used to observe the bacterial colonies more closely and to capture images of them. Four different types of colonies were identified. A total of twenty isolates from these different types were identified with specific colony morphology phenotypes. Once the different types were identified, we inoculated each of the different colonies into yeast extract, allowing it to grow again in a shaking incubator. Stock cultures are made by growing the isolates in Yeast Extract and mixing them with glycerol, and DNA is extracted using the Promega Wizard Genomic kit. The genomic DNA will be sequenced with the MinION DNA sequencing device. The genome sequences will be assembled using Trycycler and compared to the reference genome to identify mutations that determine colony morphology.
Location
Don and Karen DeRosa University Center (DUC) Poster Hall
Start Date
27-4-2024 10:30 AM
End Date
27-4-2024 12:30 PM
Genomic evaluation of morphological variation in Variovorax paradoxus EPS
Don and Karen DeRosa University Center (DUC) Poster Hall
Variovorax paradoxus is a species of versatile bacteria with a remarkable capacity for degrading organic pollutants, presenting promising opportunities for bioremediation applications. Their ability to metabolize various environmental contaminants, such as pesticides and aromatic compounds, highlights their potential role in mitigating pollution and restoring ecological balance. Understanding the mechanisms underlying their degradation pathways is crucial for harnessing their bioremediation capabilities effectively. In this research, we applied various methodologies to observe the genomic characteristics across accessible sequences of Variovorax species.
A previously grown colony was mixed into yeast extract for inoculation. After giving it time to grow in the shaking incubator for a few days, we took the liquid cultures and plated them onto freshwater medium. Allowing it to incubate, we then streaked and restreaked the colonies several times to ensure that each plate involved only one bacterial type (per plate). To determine the different colony types found amongst our plates, the Echo revolve microscope was used to observe the bacterial colonies more closely and to capture images of them. Four different types of colonies were identified. A total of twenty isolates from these different types were identified with specific colony morphology phenotypes. Once the different types were identified, we inoculated each of the different colonies into yeast extract, allowing it to grow again in a shaking incubator. Stock cultures are made by growing the isolates in Yeast Extract and mixing them with glycerol, and DNA is extracted using the Promega Wizard Genomic kit. The genomic DNA will be sequenced with the MinION DNA sequencing device. The genome sequences will be assembled using Trycycler and compared to the reference genome to identify mutations that determine colony morphology.
