Evaluation of Flavones Binding to Nucleic Acids through Competitive Dialysis
Poster Number
9A
Research or Creativity Area
Natural Sciences
Abstract
Competitive dialysis is a method in which the selective binding of ligands to nucleic acids can be quantified. This method utilizes the concept of diffusion against a semipermeable membrane to evaluate concentrations of binding. In this presentation, we report the establishment of a competition dialysis assay for studying ligand-nucleic acid interactions in the lab. For this study, an array of nucleic acids including single-stranded DNA/RNA, duplex DNA, triplex DNA, duplex RNA, triplex RNA, DNA-RNA hybrids, and G-quadruplex were dialyzed against a flavone derivative. Flavones are naturally occurring products that are found in fruits and vegetables and have been found to have antioxidative, anti-inflammatory, and anti-carcinogenic properties. The flavone derivatives were previously discovered by our lab to stabilize triplex DNA while having minimal effects on duplex DNA through thermal denaturation monitored by UV. By using competitive dialysis, we are able to see which types of nucleic acids a ligand selectively binds in a high-throughput manner. This study confirms the results of our previous thermal denaturation studies, which will allow us to do more studies focusing on the ligands that exhibit binding effects to specifically triplex DNA in the future.
Location
Don and Karen DeRosa University Center (DUC) Poster Hall
Start Date
27-4-2024 10:30 AM
End Date
27-4-2024 12:30 PM
Evaluation of Flavones Binding to Nucleic Acids through Competitive Dialysis
Don and Karen DeRosa University Center (DUC) Poster Hall
Competitive dialysis is a method in which the selective binding of ligands to nucleic acids can be quantified. This method utilizes the concept of diffusion against a semipermeable membrane to evaluate concentrations of binding. In this presentation, we report the establishment of a competition dialysis assay for studying ligand-nucleic acid interactions in the lab. For this study, an array of nucleic acids including single-stranded DNA/RNA, duplex DNA, triplex DNA, duplex RNA, triplex RNA, DNA-RNA hybrids, and G-quadruplex were dialyzed against a flavone derivative. Flavones are naturally occurring products that are found in fruits and vegetables and have been found to have antioxidative, anti-inflammatory, and anti-carcinogenic properties. The flavone derivatives were previously discovered by our lab to stabilize triplex DNA while having minimal effects on duplex DNA through thermal denaturation monitored by UV. By using competitive dialysis, we are able to see which types of nucleic acids a ligand selectively binds in a high-throughput manner. This study confirms the results of our previous thermal denaturation studies, which will allow us to do more studies focusing on the ligands that exhibit binding effects to specifically triplex DNA in the future.
