RNA-Seq differential analysis of Variovorax Paradoxus on different phases: stationary, biofilm and log phase
Abstract
Gram-negative, beta proteobacterium Variovorax paradoxus is commonly linked to biodegradative processes in nature. Because of its varied metabolic capacities, which include the biodegradation of both biogenic chemicals and anthropogenic pollutants, it is a microorganism of interest. In this project, we want to see the gene expression of the bacteria in each phase including stationary phase, biofilm and log phase and Each phase will have 3 samples for analysis. This project will use datasets from National Center for Biotechnology Information (NCBI), SRA PRJNA594416, datasets code range is from SRR10613920 to 10613928, with Kallisto library for running index transcriptome and Sleuth library to run the gene expression, so that it can be helpful for not only gene mapping, genome analysis, extraction but also the regulatory of the bacteria’s genes.
RNA-Seq differential analysis of Variovorax Paradoxus on different phases: stationary, biofilm and log phase
Don and Karen DeRosa University Center (DUC) Poster Hall
Gram-negative, beta proteobacterium Variovorax paradoxus is commonly linked to biodegradative processes in nature. Because of its varied metabolic capacities, which include the biodegradation of both biogenic chemicals and anthropogenic pollutants, it is a microorganism of interest. In this project, we want to see the gene expression of the bacteria in each phase including stationary phase, biofilm and log phase and Each phase will have 3 samples for analysis. This project will use datasets from National Center for Biotechnology Information (NCBI), SRA PRJNA594416, datasets code range is from SRR10613920 to 10613928, with Kallisto library for running index transcriptome and Sleuth library to run the gene expression, so that it can be helpful for not only gene mapping, genome analysis, extraction but also the regulatory of the bacteria’s genes.
