RNA-Seq differential analysis of Variovorax Paradoxus on different phases: stationary, biofilm and log phase

Poster Number

19C

Lead Author Affiliation

Computer Science

Lead Author Status

Masters Student

Second Author Affiliation

Computer Science

Second Author Status

Faculty

Third Author Affiliation

Biological Sciences

Third Author Status

Faculty

Research or Creativity Area

Engineering & Computer Science

Abstract

Gram-negative, beta proteobacterium Variovorax paradoxus is commonly linked to biodegradative processes in nature. Because of its varied metabolic capacities, which include the biodegradation of both biogenic chemicals and anthropogenic pollutants, it is a microorganism of interest. In this project, we want to see the gene expression of the bacteria in each phase including stationary phase, biofilm and log phase. The project will use datasets from National Center for Biotechnology Information (NCBI), SRA PRJNA594416, datasets code range is from SRR10613920 to 10613928, with Kallisto library for running index transcriptome and Sleuth library to run the gene expression. In the future, researchers may see it useful to clarify the kinetics of gene expression and metabolic fluxes in Variovorax paradoxus, create mathematical models and simulations or optimize biodegradation processes for environmental cleanup and anticipate cellular responses to environmental perturbations via systems biology techniques.

Purpose

The purpose of the project is research for the gene expression of the bacteria Variovorax Paradoxus. On the other hands, we can see how the software works in bioinformatics and contribute to the National Center for Biotechnology Information (NCBI)

Results

The result will be updated

Location

Don and Karen DeRosa University Center (DUC) Poster Hall

Start Date

27-4-2024 10:30 AM

End Date

27-4-2024 12:30 PM

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Apr 27th, 10:30 AM Apr 27th, 12:30 PM

RNA-Seq differential analysis of Variovorax Paradoxus on different phases: stationary, biofilm and log phase

Don and Karen DeRosa University Center (DUC) Poster Hall

Gram-negative, beta proteobacterium Variovorax paradoxus is commonly linked to biodegradative processes in nature. Because of its varied metabolic capacities, which include the biodegradation of both biogenic chemicals and anthropogenic pollutants, it is a microorganism of interest. In this project, we want to see the gene expression of the bacteria in each phase including stationary phase, biofilm and log phase. The project will use datasets from National Center for Biotechnology Information (NCBI), SRA PRJNA594416, datasets code range is from SRR10613920 to 10613928, with Kallisto library for running index transcriptome and Sleuth library to run the gene expression. In the future, researchers may see it useful to clarify the kinetics of gene expression and metabolic fluxes in Variovorax paradoxus, create mathematical models and simulations or optimize biodegradation processes for environmental cleanup and anticipate cellular responses to environmental perturbations via systems biology techniques.