Solid Phase Peptide Synthesis and Mass Spectrometry Analysis
Poster Number
18C
Format
Poster Presentation
Faculty Mentor Name
Dr. Jianhua Ren
Faculty Mentor Department
Chemistry Department
Additional Mentors
Ashleigh Ramos, a_ramos24@u.pacific.edu, Chemistry Department
Kim Harvey, k_harvey4@u.pacific.edu, Chemistry Department
Shiyaun Wang, s_wang27@u.pacific.edu, Chemistry Department
Abstract/Artist Statement
Introduction:
A peptide is a short chain of amino acids; longer chains are called proteins. Both are crucial for understanding their various effects on the body. Certain peptides may be used to help your body fight bacteria and boost healing. Within this lab, synthesized and analyzed were two tripeptides (CAG and PDP) with Rink Amide resin. This was carried out through the processes of deprotection, coupling, acetylation, cleavage, purification, and lyophilization.
Method:
Synthesis of the peptide begins with the SPPS vessel using rink amide resin to provide support for the formation of peptide bonds from the C-Terminal to the N-Terminal. The deprotection step removes the Fmoc protection group so that coupling can occur. Washing steps using methanol, dichloromethane (DCM) and dimethylformaide (DMF) throughout the experiment remove any waste for couplings. Using the desired amino acid, HBTU, DMF, and DIPEA the amino acid can be attached. These steps are repeated until the desired peptide is synthesized. Once synthesized, cleavage removes the Fmoc protection group and peptide from the rink amide resin, where it is then acetylated to replace the N-Terminal with an acetyl group. Finally the product is analyzed using mass spectrometry to determine its purity.
Result:
By following this procedure, the desired tripeptides were successfully synthesized and evaluated for accuracy.
Location
Information Commons, William Knox Holt Memorial Library and Learning Center
Start Date
29-4-2023 10:00 AM
End Date
29-4-2023 1:00 PM
Solid Phase Peptide Synthesis and Mass Spectrometry Analysis
Information Commons, William Knox Holt Memorial Library and Learning Center
Introduction:
A peptide is a short chain of amino acids; longer chains are called proteins. Both are crucial for understanding their various effects on the body. Certain peptides may be used to help your body fight bacteria and boost healing. Within this lab, synthesized and analyzed were two tripeptides (CAG and PDP) with Rink Amide resin. This was carried out through the processes of deprotection, coupling, acetylation, cleavage, purification, and lyophilization.
Method:
Synthesis of the peptide begins with the SPPS vessel using rink amide resin to provide support for the formation of peptide bonds from the C-Terminal to the N-Terminal. The deprotection step removes the Fmoc protection group so that coupling can occur. Washing steps using methanol, dichloromethane (DCM) and dimethylformaide (DMF) throughout the experiment remove any waste for couplings. Using the desired amino acid, HBTU, DMF, and DIPEA the amino acid can be attached. These steps are repeated until the desired peptide is synthesized. Once synthesized, cleavage removes the Fmoc protection group and peptide from the rink amide resin, where it is then acetylated to replace the N-Terminal with an acetyl group. Finally the product is analyzed using mass spectrometry to determine its purity.
Result:
By following this procedure, the desired tripeptides were successfully synthesized and evaluated for accuracy.