Synthesis of Substituted Flavone Derivatives for Triplex Binding
Poster Number
19C
Format
Poster Presentation
Faculty Mentor Name
Liang Xue
Faculty Mentor Department
Chemistry Department
Graduate Student Mentor Name
Nghia Tran
Graduate Student Mentor Department
Chemistry Department
Additional Mentors
Landy Gu, l_gu2@u.pacific.edu, Chemistry Department
Vanessa Rangel, v_rangel1@u.pacific.edu, Chemistry Department
Abstract/Artist Statement
Triplex DNA is a DNA structure composed of three strands of DNA. Triplexes can form intramolecularly and intermolecularly. Intramolecular triplex formation occurs when a DNA strand folds on itself and forms Hoogsteen H-bonds. Intermolecular triplex formation occurs when a third external strand is introduced and binds to the duplex, and forms Hoogsteen bonds. This third strand is known as a triplex-forming oligonucleotide (TFO). Triplex research has become more relevant because of its potential for antigene therapy. The binding of TFOs to specific regions of the DNA can alter the transcription and translation of certain genes. This can be used to silence certain regions of the DNA responsible for genetic diseases and even cancer. Previously, our group found that 5-substituted quercetin derivatives strongly bind to triplex DNA while having minimal impact on duplex DNA. In this report, I will present the synthesis of quercetin derivatives with an amino-containing side chain at different positions and evaluate their ability to stabilize triplex DNA using thermal denaturation monitored by UV.
Location
Information Commons, William Knox Holt Memorial Library and Learning Center
Start Date
29-4-2023 10:00 AM
End Date
29-4-2023 1:00 PM
Synthesis of Substituted Flavone Derivatives for Triplex Binding
Information Commons, William Knox Holt Memorial Library and Learning Center
Triplex DNA is a DNA structure composed of three strands of DNA. Triplexes can form intramolecularly and intermolecularly. Intramolecular triplex formation occurs when a DNA strand folds on itself and forms Hoogsteen H-bonds. Intermolecular triplex formation occurs when a third external strand is introduced and binds to the duplex, and forms Hoogsteen bonds. This third strand is known as a triplex-forming oligonucleotide (TFO). Triplex research has become more relevant because of its potential for antigene therapy. The binding of TFOs to specific regions of the DNA can alter the transcription and translation of certain genes. This can be used to silence certain regions of the DNA responsible for genetic diseases and even cancer. Previously, our group found that 5-substituted quercetin derivatives strongly bind to triplex DNA while having minimal impact on duplex DNA. In this report, I will present the synthesis of quercetin derivatives with an amino-containing side chain at different positions and evaluate their ability to stabilize triplex DNA using thermal denaturation monitored by UV.