Title

Creating a video about how to perform a spot western assay to identify successful expression strains of the yeast Pichia pastoris

Lead Author Major

Pre-dentistry and Biological Sciences

Lead Author Status

Sophomore

Second Author Major

Pre-dentistry and Biological Sciences

Second Author Status

Junior

Third Author Major

Pre-dentistry and Biological Sciences

Third Author Status

Senior

Fourth Author Major

Pre-dentistry and Biological Sciences

Fourth Author Status

Sophomore

Fifth Author Major

Pre-Pharmacy

Fifth Author Status

Junior

Format

Oral Presentation

Faculty Mentor Name

Geoff Lin-Cereghino

Faculty Mentor Department

Biological Sciences

Additional Faculty Mentor Name

Joan Lin-Cereghino

Additional Faculty Mentor Department

Biological Sciences

Abstract/Artist Statement

The yeast Pichia pastoris is used as a host to produce valuable recombinant proteins such as insulin and COVID 19 vaccine antigens. One challenge in using this yeast is to identify the strain that expresses the highest amount of the desired protein. Our lab had devised a procedure called the spot western assay which enables a scientist to screen many strains in a rapid, efficient manner. Protein samples are spotted onto a membrane and then bound by antibodies to detect the amount of the desired recombinant protein. Up to 60 samples can be screened in less than 2 hours with this procedure. We have created a video which describes how to perform the spot western assay, which we will share through our YouTube channel (Pichia pastoris Saves the World https://www.youtube.com/channel/UCkG51_QRep7aWnis4Fp98Xw). We believe that this technique will encourage scientists to try using this recombinant yeast as well as accelerate the progress of experienced Pichia pastoris users worldwide.

Location

Sierra Learning Lab, William Knox Holt Memorial Library and Learning Center

Start Date

30-4-2022 10:40 AM

End Date

30-4-2022 10:59 AM

This document is currently not available here.

Share

COinS
 
Apr 30th, 10:40 AM Apr 30th, 10:59 AM

Creating a video about how to perform a spot western assay to identify successful expression strains of the yeast Pichia pastoris

Sierra Learning Lab, William Knox Holt Memorial Library and Learning Center

The yeast Pichia pastoris is used as a host to produce valuable recombinant proteins such as insulin and COVID 19 vaccine antigens. One challenge in using this yeast is to identify the strain that expresses the highest amount of the desired protein. Our lab had devised a procedure called the spot western assay which enables a scientist to screen many strains in a rapid, efficient manner. Protein samples are spotted onto a membrane and then bound by antibodies to detect the amount of the desired recombinant protein. Up to 60 samples can be screened in less than 2 hours with this procedure. We have created a video which describes how to perform the spot western assay, which we will share through our YouTube channel (Pichia pastoris Saves the World https://www.youtube.com/channel/UCkG51_QRep7aWnis4Fp98Xw). We believe that this technique will encourage scientists to try using this recombinant yeast as well as accelerate the progress of experienced Pichia pastoris users worldwide.