Creating a video about how to perform a spot western assay to identify successful expression strains of the yeast Pichia pastoris
Format
Oral Presentation
Faculty Mentor Name
Geoff Lin-Cereghino
Faculty Mentor Department
Biological Sciences
Additional Faculty Mentor Name
Joan Lin-Cereghino
Additional Faculty Mentor Department
Biological Sciences
Abstract/Artist Statement
The yeast Pichia pastoris is used as a host to produce valuable recombinant proteins such as insulin and COVID 19 vaccine antigens. One challenge in using this yeast is to identify the strain that expresses the highest amount of the desired protein. Our lab had devised a procedure called the spot western assay which enables a scientist to screen many strains in a rapid, efficient manner. Protein samples are spotted onto a membrane and then bound by antibodies to detect the amount of the desired recombinant protein. Up to 60 samples can be screened in less than 2 hours with this procedure. We have created a video which describes how to perform the spot western assay, which we will share through our YouTube channel (Pichia pastoris Saves the World https://www.youtube.com/channel/UCkG51_QRep7aWnis4Fp98Xw). We believe that this technique will encourage scientists to try using this recombinant yeast as well as accelerate the progress of experienced Pichia pastoris users worldwide.
Location
Sierra Learning Lab, William Knox Holt Memorial Library and Learning Center
Start Date
30-4-2022 10:40 AM
End Date
30-4-2022 10:59 AM
Creating a video about how to perform a spot western assay to identify successful expression strains of the yeast Pichia pastoris
Sierra Learning Lab, William Knox Holt Memorial Library and Learning Center
The yeast Pichia pastoris is used as a host to produce valuable recombinant proteins such as insulin and COVID 19 vaccine antigens. One challenge in using this yeast is to identify the strain that expresses the highest amount of the desired protein. Our lab had devised a procedure called the spot western assay which enables a scientist to screen many strains in a rapid, efficient manner. Protein samples are spotted onto a membrane and then bound by antibodies to detect the amount of the desired recombinant protein. Up to 60 samples can be screened in less than 2 hours with this procedure. We have created a video which describes how to perform the spot western assay, which we will share through our YouTube channel (Pichia pastoris Saves the World https://www.youtube.com/channel/UCkG51_QRep7aWnis4Fp98Xw). We believe that this technique will encourage scientists to try using this recombinant yeast as well as accelerate the progress of experienced Pichia pastoris users worldwide.