Overcoming Obstacles in Pichia pastoris Protein Expression: The Article and the Movie
Format
Oral Presentation
Faculty Mentor Name
Geoff Lin-Cereghino
Faculty Mentor Department
Department of Biological Sciences
Additional Faculty Mentor Name
Joan Lin-Cereghino
Additional Faculty Mentor Department
Department of Biological Sciences
Abstract/Artist Statement
The yeast Pichia pastoris (also known as Komagataella pastoris) has been used for about 30 years to produce thousands of recombinant proteins, such as insulin to treat diabetes and antibodies to prevent migraines. Despite its success, there are some stubborn problems encountered by research scientists when they try to use Pichia pastoris to produce their recombinant proteins. In order to provide those working in this field with strategies to overcome these common obstacles, we interviewed eight experts in Pichia protein expression to create a written review and video. The article and video contain a collection of short narratives describing how each featured scientist addressed a specific problem, focusing on his/her strategy, key achievements, and the long term effects of the work. The video will be previewed publicly for the first time during this PURCC presentation, and we will be available for any comments and questions. We expect that our work will be a tool to help empower scientists to successfully express challenging proteins in this popular yeast.
Location
University of the Pacific, 3601 Pacific Ave., Stockton, CA 95211
Start Date
24-4-2021 3:45 PM
End Date
24-4-2021 4:00 PM
Overcoming Obstacles in Pichia pastoris Protein Expression: The Article and the Movie
University of the Pacific, 3601 Pacific Ave., Stockton, CA 95211
The yeast Pichia pastoris (also known as Komagataella pastoris) has been used for about 30 years to produce thousands of recombinant proteins, such as insulin to treat diabetes and antibodies to prevent migraines. Despite its success, there are some stubborn problems encountered by research scientists when they try to use Pichia pastoris to produce their recombinant proteins. In order to provide those working in this field with strategies to overcome these common obstacles, we interviewed eight experts in Pichia protein expression to create a written review and video. The article and video contain a collection of short narratives describing how each featured scientist addressed a specific problem, focusing on his/her strategy, key achievements, and the long term effects of the work. The video will be previewed publicly for the first time during this PURCC presentation, and we will be available for any comments and questions. We expect that our work will be a tool to help empower scientists to successfully express challenging proteins in this popular yeast.