Investigation of the Binding of Aminoglycosides to c-Myc G-quadruplex DNA Using the FID Assay
Poster Number
18B
Format
Poster Presentation
Faculty Mentor Name
Liang Xue
Faculty Mentor Department
Chemistry
Graduate Student Mentor Name
Vanessa Rangel
Graduate Student Mentor Department
Chemistry
Abstract/Artist Statement
More than 300,000 guanine-rich sequences are present in the human genome, many of which can form a unique DNA secondary structure known as G-quadruplex (G4). The formation of G-quadruplex structure has been confirmed in vivo, arousing much attention on their potential structure and function relationship. G-quadruplexes are believed to block the binding of DNA-processing enzymes and subsequently regulate their biological functions. Many small molecules, known as G4 ligands, can facilitate the formation of G-quadruplexes, which have been recognized as potential drugs for the treatment of various diseases. Our previous studies of a class of antibiotics (aminoglycosides) suggested that neomycin can efficiently bind to telomeric G- quadruplex DNA. In this study, we further investigated the binding of aminoglycosides to a G-quadruplex structure formed in the promoter region of an oncogene (c-Myc) using the FID assay. Our results suggest that amongst the nine aminoglycosides tested, neomycin has the best binding affinity toward c-Myc G-quadruplex DNA. The apparent strong binding of neomycin with G-quadruplex DNA probably results from its molecular shape and multiple positive charges.
Location
DeRosa University Center Ballroom
Start Date
27-4-2018 10:00 AM
End Date
27-4-2018 12:00 PM
Investigation of the Binding of Aminoglycosides to c-Myc G-quadruplex DNA Using the FID Assay
DeRosa University Center Ballroom
More than 300,000 guanine-rich sequences are present in the human genome, many of which can form a unique DNA secondary structure known as G-quadruplex (G4). The formation of G-quadruplex structure has been confirmed in vivo, arousing much attention on their potential structure and function relationship. G-quadruplexes are believed to block the binding of DNA-processing enzymes and subsequently regulate their biological functions. Many small molecules, known as G4 ligands, can facilitate the formation of G-quadruplexes, which have been recognized as potential drugs for the treatment of various diseases. Our previous studies of a class of antibiotics (aminoglycosides) suggested that neomycin can efficiently bind to telomeric G- quadruplex DNA. In this study, we further investigated the binding of aminoglycosides to a G-quadruplex structure formed in the promoter region of an oncogene (c-Myc) using the FID assay. Our results suggest that amongst the nine aminoglycosides tested, neomycin has the best binding affinity toward c-Myc G-quadruplex DNA. The apparent strong binding of neomycin with G-quadruplex DNA probably results from its molecular shape and multiple positive charges.