Examining the Interaction of the T. vaginalis homologues of RAD51 with human Hela Library
Poster Number
08B
Format
Poster Presentation
Faculty Mentor Name
Lisa Wrischnik
Faculty Mentor Department
Biological Sciences
Abstract/Artist Statement
Tricohomonus vaginalis is a protozoa that causes vaginitis, with around 280 million cases each year worldwide. This sexually transmitted disease causes noxious discharge, irritation of the vagina, and results in premature birth in pregnant women, and may lead to susceptibility to other diseases. The T. vaginalis genome contains genes responsible for meiotic recombination even though T. vaginalis has not been seen to have a sexual stage. Our role is to try to understand the function of two of these genes, Rad51 and DMC1, which act during recombination. After confirming interactions between both RAD51 and DMC with a T. vaginalis BRCA2 homolog in directed yeast 2-hybrid analysis last year, this year’s goal was to find new binding partners with RAD51. To do this, we conducted a Yeast 2-Hybrid Library Screen with Rad51 as the “bait” and a HeLa cell library as “prey”. After mating the bait and library strains, any colonies that survived indicated a positive interaction between Rad51 and the unknown “prey” protein from the library. The genes encoding the “prey” were isolated through PCR, gel purified and sent to be sequenced. The intention of this research is to find potential binding partners for Rad51 to help us hypothesize about the function of RAD51 in T. vaginalis, and results of the screen will be discussed.
Location
DeRosa University Center, Ballroom
Start Date
29-4-2017 1:00 PM
End Date
29-4-2017 3:00 PM
Examining the Interaction of the T. vaginalis homologues of RAD51 with human Hela Library
DeRosa University Center, Ballroom
Tricohomonus vaginalis is a protozoa that causes vaginitis, with around 280 million cases each year worldwide. This sexually transmitted disease causes noxious discharge, irritation of the vagina, and results in premature birth in pregnant women, and may lead to susceptibility to other diseases. The T. vaginalis genome contains genes responsible for meiotic recombination even though T. vaginalis has not been seen to have a sexual stage. Our role is to try to understand the function of two of these genes, Rad51 and DMC1, which act during recombination. After confirming interactions between both RAD51 and DMC with a T. vaginalis BRCA2 homolog in directed yeast 2-hybrid analysis last year, this year’s goal was to find new binding partners with RAD51. To do this, we conducted a Yeast 2-Hybrid Library Screen with Rad51 as the “bait” and a HeLa cell library as “prey”. After mating the bait and library strains, any colonies that survived indicated a positive interaction between Rad51 and the unknown “prey” protein from the library. The genes encoding the “prey” were isolated through PCR, gel purified and sent to be sequenced. The intention of this research is to find potential binding partners for Rad51 to help us hypothesize about the function of RAD51 in T. vaginalis, and results of the screen will be discussed.