Expression of Fluorescently Tagged Proteins in Pichia pastoris
Poster Number
34
Format
Poster Presentation
Faculty Mentor Name
Joan Lin-Cereghino
Faculty Mentor Department
Biological Sciences
Additional Faculty Mentor Name
Geoffrey Lin-Cereghino
Abstract/Artist Statement
The aim of this study was to recombinantly express Pyriform Spidroin 1 (PySp1) and Protein Kinase C (PKC1) in the yeast Pichia pastoris. PySp1 is a spider silk protein that we believe can be expressed and secreted in large amounts by Pichia pastoris for potential industrial and medical purposes. Because PKC1 is involved in intracellular signaling, the localization of PKC1 in Pichia pastoris could provide a better understanding of the ability of Pichia pastoris to super secrete. EGFP fusion proteins were quantified and analyzed by Western Blot and fluorescent microscopy.
Location
DeRosa University Center, Ballroom
Start Date
26-4-2014 2:00 PM
End Date
26-4-2014 4:00 PM
Expression of Fluorescently Tagged Proteins in Pichia pastoris
DeRosa University Center, Ballroom
The aim of this study was to recombinantly express Pyriform Spidroin 1 (PySp1) and Protein Kinase C (PKC1) in the yeast Pichia pastoris. PySp1 is a spider silk protein that we believe can be expressed and secreted in large amounts by Pichia pastoris for potential industrial and medical purposes. Because PKC1 is involved in intracellular signaling, the localization of PKC1 in Pichia pastoris could provide a better understanding of the ability of Pichia pastoris to super secrete. EGFP fusion proteins were quantified and analyzed by Western Blot and fluorescent microscopy.